The carob (Ceratonia siliqua L.) is very old edible fruit, flavorful and often used in foods and beverages. In this present study the composition of phenolic compounds, the antioxidant activity and the production locust bean gum (LBG) of seeds grown in Algeria was explored.
The phenolic contents (total phenolics, total tannins, total flavonoids, condensed tannins and flavonols) and the antioxidant activity of the aqueous methanolic, ethanolic and acetonic extracts (70, 80 and 80v/v, respectively) of the seeds were determined using colorimetric methods. Furthermore, the carob bean gum from seeds was extracted and its chemical composition was evaluated.
Aqueous acetonicextract of carob seeds give the highest contents of total phenolics, total flavonoids, flavonols, total tannins and condensed tannins which were 12.24±0.02, 1.33±0.01, 2.97±0.01, 4.29±0.2 and 0.53±0.01 mg/g of dry weight, respectively. The same extract showed also the best antioxidant activity in all assays compared to other extracts.
Battle I, Tous J. Carob tree (Ceratonia siliqua L.). Promoting the conservation and use of under-utilised and neglected crops. Roma: Institute of Plant Genetics and Crop Plant Research; Gatersleben: International Plant Genetic Resource Institute (IPGRI), 1997.
Kıvrak NE, Aşkın B, Küçüköner E. Comparison of some physicochemical properties of locust bean seeds gum extracted by acid and water pre-treatments. Food Nutr Sci. 2015;6:278–6.
TheophilouI C, Neophytou CM, Kakas A, Constantinou AI. Carob and its component in the management of gastrointestinal disorders. J HepatolGastroenterol. 2017;1:005.
Avallone R, Plessi M, Baraldi M, Monzani A. Determination of chemical composition of carob (Ceratonia siliqua): protein, fat, carbohydrates, and tannins. J Food Comp Anal. 1997;10:166.
Dakia PA, Wathelet B, Paquot M. Isolation and chemical evaluation of carob (Ceratonia siliqua L.). Seed Germ Food Chem. 2007;102:1368–74.
Makris DP, Kefalas P. Carob pods (Ceratonia siliqua L.) as a sources of polyphenolic antioxidants. Food Technol Biotechnol. 2004;42:105–8.
Custodio L, Fernandes E, Escapa AL, Fajardo A, Aligue R, Albericio F, et al. Antioxidant and cytotoxic activities of carob tree fruit pulps are strongly influenced by gender and cultivar. J Agric Food Chem. 2011;59:7005–12.
Durazzo A, Turfani V, Narducci V, Azzini E, Maiani G, Carcea M. Nutritional characterisation and bioactive components of commercial carobs flour. Food Chem. 2014;153:109–13.
Sanchez M, Franco D, Sineiro J, Magarinos B, Nunez MJ. Antioxidant power, bacteriostatic activity, and characterization of white grape pomace extracts by HPLC–ESI–MS. Eur Food Res Technol. 2009;230:291–01.
Bahorun T, Luximon-Ramma A, Crozier A, Okezie IA. Total phenol, flavonoid, proanthocyanidin and vitamin C levels and antioxidant activities of Mauritian vegetables. J Sci Food Agri. 2004;84:1553–6.
Kumaran A, Karunakaran JR. In-vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT Food Sci Technol. 2007;40:344.
Hagerman AE, Butler LG. Protein precipitation method for the quantitative determination of tannins. Agri Food Chern. 1978;4:809–12.
Qian H, Nihorimbere V. Antioxidant power of phytochemicals from Psidium guajava leaf. J Zhejiang Univ Sci. 2004;5:676–83
Huang YC, Chang YH, Shao YY. Effects of genotype and treatment on the antioxidant activity of sweet potato in Taiwan. Food Chem. 2006;98:529–38.
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E1. E Anal Biochem. 1999;269:337–41.
AOAC. Official methods of analysis, 16th ed. Virginia, USA: Association of Official Analytical Chemists International Arligton, 2000.
Dubois M, Gilles KA, Hamilton JK, Rebers PT, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem. 1956;28:350–6.
PaduaM D, GroworskiFountoura PS, Mathias AL. Chemical composition of Ulvariaoxysperma(Kützing) bliding, Ulva lactuca (Linnaeus) and Ulva fascita (Delile). Braz Arch Biol Technol. 2004;47:49–5.
AOAC. Official methods of analysis of AOAC international. Gaithersburg, Maryland: AOAC, 2006.
Cork SJ, Krockenberger AK. Methods and pitfalls of extracting condensed tannins and other phenolics from plants: insights from investigations on Eucalyptus leaves. J Chem Ecol. 1991;17:123–34.
Nazck M, Shahidi F. Phenolics in cereals, fruits and vegetables: occurrence, extraction and analysis. J Pharma Biomed Anal. 2006;41:1523–42.
Papagiannopoulos M, Wollseifen HR, Mellenthin A, Haber B, Galensa R. Identification and quantification of polyphenols in carob fruits (Ceratonia Siliqua L.) and derived products by HPLC – UV – ESI/MS. J Agri Food Chem. 2004;52:3784–91.
Da Silva JAL, Gonçalves MP. Studies on a purification method for locust bean gum by precipitation with isopropanol. Food Hydrocoll. 1990;4:277–87.
Andrade C, Azero E, Luciano L, Gonçalves M. Solution properties of the galactomannans extracted from the seeds of Caesalpiniapulcherrima and Cassia javanica: comparison with locust bean gum. Int J Biol Macromol. 1999;26:181–5.
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