Cytogenetic impact of sodium chloride stress on root cells of Vigna radiata L. seedlings

  • 1 Department of Genetics, Faculty of Science, University of Karachi, Karachi 75270, Pakistan
  • 2 Department of Genetics, Faculty of Science, University of Karachi, Karachi, Pakistan
Jabeen FarheenORCID iD: and Simeen Mansoor
  • Department of Genetics, Faculty of Science, University of Karachi, Karachi, Pakistan
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The high salinization stress to seedling is the substantial ecological problem in the ongoing era. It negatively influences the growth that retard mitotic division by enhancing aberrations in nuclear chromatin. In the light of these views, the current work was designed to investigate the response of Vigna seedlings root tip cells to the presence of NaCl ions.

Materials and methods

NM-92 and NM19-19 seeds were imbibed separately in distilled water for 24 h and allowed to grow into 0, 50, 150, 250, and 350 mM NaCl solution for 24 h. Excised root tips were stained, and slides were scored at 100× objective for the mitotic index (MI) and chromosomal aberrations.


Our data demonstrated that as NaCl molarity increased, the MI was declined along with various chromatin abnormalities. The 150 mM of NaCl showed more lagging (69%) of chromosomes during anaphase in NM19-19. The highest stickiness at metaphase stage (68%) was found in 250 mM NaCl in variety NM19-19. However, both varieties were differed non-significantly for c-mitosis that was recorded 99% at 350 mM NaCl concentration.


The NaCl ions toxicity induced various cytological anomalies in seedling roots that adversely affect the growth of Vigna seedlings.

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Turkish Journal of Biochemistry (TJB), official journal of Turkish Biochemical Society, is issued electronically every 2 months. The main aim of the journal is to support the research and publishing culture by ensuring that every published manuscript has an added value and thus providing international acceptance of the “readability” of the manuscripts published in the journal.