Photochemie von Ribonucleinsäuren

I. Die Photoprodukte von Uridylsäure und Cytidylsäure und ihr Nachweis in UV-bestrahlten Ribonucleinsäuren

Heinz Schuster 1
  • 1 Aus dem Max-Planck-Institut für Virusforschung, Tübingen

Irradiation of uridylic acid (Up) in buffer (at 254 mµ or 280 mµ) yields the hydrated molecule (UpH2O), which on subsequent alkaline treatment decomposes to Up (65%) and a new compound A (35%) with the pyrimidine-ring opened. Irradiation in ice (254 mµ) leads to the formation of

the uridylic acid dimer (Up/Up), which on re-irradiation reverts to Up (230 mµ, in neutral solution) or compound A, via Up and UpH2O) (254 mµ, in alkaline solution).

Similarly the hydrated cytidylic acid (CpH2O) is formed by irradiation of cytidylic acid (Cp) in buffer (254 mµ). In a subsequent dark reaction most of the CpH2O-molecules revert to Cp, a minor part being deaminated to UpH2O. Alkaline treatment of an irradiated Cp-solution yields Cp, Up and compound A, the latter two arising by decomposition of UpH2O. Neither Cp nor Up dimers were found after irradiation of Cp in ice (254 mµ).

All the photoproducts of Up and Cp, in which the pyrimidine-ring was still preserved, lost 1 - 3% of their phosphoric acid (Pi) under the experimental conditions.

The alkaline decomposition of UpH2O is used as a method for the titration of hydrated uracil residues in irradiated ribonucleic acid (RNA). Following irradiation (254 mµ) the RNA is degraded by alkali completely to mononucleotides and three new photoproducts: compound A, ribose phosphate, and another compound B, which behaves like Up dimer. Form the amount of compound A found, the number of hydrated uracils originally formed can be calculated. Furthermore the kinetics of the formation of the photoproducts are studied.

The biological implications of these studies are discussed.

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