Über den Katabolismus von Hamamelose [2-C(Hydroxymethyl)-ᴰ-ribose] / On the Catabolism of Hamamelose [2-C-(hydroxymethyl)-ᴰ-ribose]

I. Die Oxidation von Hamamelose durch Pseudomonas ] / I. The oxidation of Hamamelose by Pseudomonas

A. Thanbichler 1 , H. Gilck 1  and E. Beck 1
  • 1 Botanisches Institut der Universität München, D-8000 München 19, Menzingerstraße 67

Free hamamelose [2-C- (hydroxymethyl) -ᴰ-ribose] occurs in almost all higher plants, but except a reduction to the corresponding sugar alcohol (hamamelitol) , no metabolism of this branched chain hexose could be detected in plants until now. Therefore we tried to find microorganisms which would allow us to study the catabolism of hamamelose. A strain of Pseudomonas (“H 1”), showing dependence between growth and hamamelose concentration in the medium (Fig. 1), was isolated from soil on which Primula clusiana was grown (this plant contains large amounts of hamamelose and hamamelitol). However, this organism needs citrate besides hamamelose for growth.

When “H 1” was incubated in a phosphate buffer containing only 14C-labelled hamamelose, hamamelonic acid was the sole radioactive product formed (Tables 2, 3, 4).

As no further degradation of hamamelonic acid by “H 1” could be detected, we conclude that this organism uses hamamelose as a hydrogen source only. Thus it becomes reasonable that “H 1” needs an additional carbon source (citrate) for growth.

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Zeitschrift für Naturforschung B is an international scientific journal which publishes original papers, microreviews, and letters from all areas of inorganic chemistry, solid state chemistry, coordination chemistry, molecular chemistry, and organic chemistry.