1) Proliferation and energy metabolism of in vitro grown Ehrlich ascites tumor (EAT) cells in the presence of glucosone, (ᴅ-arabino-220.127.116.11-tetrahydroxy-2-oxo-hexanal) a competitive inhibitor of hexokinase, were studied.
2) Proliferation of the cells was completely inhibited by 2 mᴍ glucosone without severely affecting viability (dye exclusion test). No phase specific arrest of cell growth was observed.
3) Incorporation of [14C]thymidine into an acid insoluble fraction of the cells decreases to 5% of the controls within 8 -10 h . Incorporation of [14C]leucine begins to slow down immediately after treatment with glucosone.
4) The inhibitor (2 mᴍ) reduces the lactate production of the cells by 60%, respiration by about 20%; the ATP/ADP ratio slows down from 4.75 to 3.5.
5) The total inhibition of cell proliferation by 2 mᴍ glucosone cannot be explained exclusively by inhibition of hexokinase activity and impairment of energy metabolism. Because of a lack of specificity, glucosone is not a suitable inhibitor for studies on the relationship between hexokinase activity and cell proliferation of tumor cells
A Journal of Biosciences: Zeitschrift für Naturforschung C (ZNC) is an international scientific journal for the emerging field of natural and natural-like products. ZNC publishes original research on the isolation, bio-chemical synthesis and bioactivities of natural products, their biochemistry, pharmacology, biotechnology, and biological activity and innovative developed computational methods for predicting their structure and/or function.