Effect of CDP-choline on Hippocampal Acetylcholinesterase and Na+,K+-ATPase in Adult and Aged Rats

Christos Plataras 1 , Panagoula Angelogianni 1  and Stylianos Tsakiris 1
  • 1 Department of Experimental Physiology, Medical School, University of Athens, P. O. Box 65257, GR-154 01 Athens, Greece

The aim of this study was to investigate the effect of different cytidine-5′-diphosphocholine (CDP-choline) concentrations (0.1-1 mm) on acetylcholinesterase (AChE), (Na+,K+)-ATPase and Mg2+-ATPase activities in homogenates of adult and aged rat hippocampi. Tissues were homogenised, centrifuged at 1000 × g for 10 min and in the supernatant, AChE activity and Na+,K+-ATPase and Mg2+-ATPase activities were determined according to Ellman’s method and Bowler’s and Tirri’s method, respectively. After an 1-3 h preincubation of the homogenised tissue with CDP-choline, a maximal AChE stimulation of about 25% for both adult and aged rats (p < 0.001) and a Na+,K+-ATPase activation of about 50% for adult rats (p < 0.001) and about 60% for aged rats (p < 0.001) were observed, while hippocampal Mg2+- ATPase activity was not influenced in either adult or aged animals. It is suggested that: CDP-choline can restore hippocampal AChE and Na+,K+-ATPase activities in the aged rat and thus it may play a role in improving memory performance which is impaired by aging and some neuronal disturbances.

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A Journal of Biosciences: Zeitschrift für Naturforschung C (ZNC) is an international scientific journal for the emerging field of natural and natural-like products. ZNC publishes original research on the isolation, bio-chemical synthesis and bioactivities of natural products, their biochemistry, pharmacology, biotechnology, and biological activity and innovative developed computational methods for predicting their structure and/or function.