Immunomodulating Polysaccharide Fractions of Menyanthes trifoliata L.

Janina Kuduk-Jaworska 1 , Joanna Szpunar 2 , Kazimierz Gąsiorowski 3 ,  and Barbara Brokos 3
  • 1 Faculty of Chemistry, Wrocław University, F. Joliot-Curie 14 str., 50-383 Wrocław, Poland.
  • 2 CNRS, EP 132, Hélioparc, 64053 Pau, France
  • 3 Department of Pharmacy, Medical University, Department of Basic Medical Sciences, Wrocław, Poland


Looking for new plant sources of immunomodulating agents polysaccharide-rich fractions (PS) from Menyanthes trifoliata L. (Menyanthaceae) have been isolated. The herb of Menyanthes trifoliata L. was sequentially extracted with water, 0.1 м NaOH, 8% CH3COOH, and 1 м NaOH. After dialysis and resolution on Biogel P-10 four homogenic (B-4, B-5, C-4, D-5) and two nonhomogenic (A-3 and D-4) PS were isolated. About 0.5% of PS over 3500 Da were found in the dry plant material. They were characterized through chemical analysis, NMR and vibrational spectroscopy. Speciation analysis of chosen metal/metaloid elements was performed and an exceptionally high concentration of Se was found in PS of a pure water extract (A-3). The biological tests on the immunomodulating influence with human blood-derived lymphocytes and granulocytes revealed that two fractions, B-4 and B-5, were strong stimulators of immune cells, whereas fractions D-5 and A-3 were found as potent suppressive and anti-inflammatory agents. The applied isolation procedures led to the separation of active compounds into stimulatory and inhibitory fractions.

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A Journal of Biosciences: Zeitschrift für Naturforschung C (ZNC) is an international scientific journal for the emerging field of natural and natural-like products. ZNC publishes original research on the isolation, bio-chemical synthesis and bioactivities of natural products, their biochemistry, pharmacology, biotechnology, and biological activity and innovative developed computational methods for predicting their structure and/or function.