Flavonoids in Helichrysum pamphylicum Inhibit Mammalian Type I DNA Topoisomerase

Zeki Topcu 1 , Bintug Ozturk 2 , Ozlem Kucukoglu 3  and Emrah Kilinc 4
  • 1 Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Ege University, 35100 Izmir, Turkey
  • 2 Pharmaceutical Botany, Faculty of Pharmacy, Ege University, 35100 Izmir, Turkey
  • 3 Pharmaceutical Toxicology, Faculty of Pharmacy, Ege University, 35100 Izmir, Turkey
  • 4 Analytical Chemistry, Faculty of Pharmacy, Ege University, 35100 Izmir, Turkey

DNA topoisomerases are important targets for cancer chemotherapy. We investigated the effects of a methanolic extract of Helichrysum pamphylicum on mammalian DNA topoisomerase I via in vitro plasmid supercoil relaxation assays. The extracts manifested a considerable inhibition of the enzyme’s activity in a dose-dependent manner. We also performed a HPLC analysis to identify the flavonoid content of the H. pamphylicum extract and tested the identified flavonoids; luteolin, luteolin-4-glucoside, naringenin, helichrysinA and isoquercitrin, on DNA topoisomerase I activity. The measurement of the total antioxidant capacity of the flavonoid standards suggested that the topoisomerase inhibition might be correlated with the antioxidant capacity of the plant.

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A Journal of Biosciences: Zeitschrift für Naturforschung C (ZNC) is an international scientific journal for the emerging field of natural and natural-like products. ZNC publishes original research on the isolation, bio-chemical synthesis and bioactivities of natural products, their biochemistry, pharmacology, biotechnology, and biological activity and innovative developed computational methods for predicting their structure and/or function.

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