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Multiphoton Microscopy and Fluorescence Lifetime Imaging

Applications in Biology and Medicine

Ed. by König, Karsten

With contrib. by Baldeweck, Thérèse / Balu, Mihaela / Batista, Ana / Vecker, Wolfgang / Belousov, Vsevolod V / Breinig, Hans Georg / Breymayer, Jasmin / Brizion, Sébastien / Castello, Marco / Chandra, Dhyan / Choi, Heejin / Decencière, Etienne / Diaspro, Alberto / Donitzky, Christoph / Dudenkova, Varvara V. / Druzhkova, Irina N. / Harris, Ronald M. / Hoffman, Robert M. / Holmes, Amy / Huck, Volker / Joshi, Suchitra / Kaiser, Wolfgang / Kalinina, Sviatlana / Kantelhardt, Sven R. / Kapur, Jaideep / Kelly, Kristen M. / König, Karten / Koudoro, Serge / Krueger, Arnd / Lanzano, Luca / Liang, Xiaowen / Liu, Xin / Lukina, Maria M. / Mascaro, Anna Letizia Allegra / Melia, Meghan J.O. / Mess, Christian / Mishina, Natalie M. / Pavone, Francesco S. / Periasamy, Ammasi / Rehman Alam, Shagufta / Roberts, Michael S. / Pena, Ana-Maria / Rowlands, Christoph / Rück, Angelika / Sacconi, Leonardo / Scipioni, Lorenzo / Sergeeva, Tatiana F. / Shcheslavskiy, Vladislav I. / Shirmanova, Marina V. / Silvestri, Ludovico / So, Peter T.C. / Studier, Hauke / Svindrych, Zdenek / Tancrede-Bohin, Emmanuelle / Thorling, Camilla / Tromberg, Bruce j. / Uchugonova, Aisada / Vicidomini, Giuseppe / Victorin, Steeve / Wallrabe, Horst / Wang, Haolu / Weinigel, Martin / Yew, Elijah / Zachary, Christopher B. / Zagaynova, Elena V.

Open Access

    Open Access
    eBook (PDF)
    Publication Date:
    January 2018
    Copyright year:
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    • Focuses on nonlinear microscopy methods
    • Starts with a tutorial chapter
    • Combines treatment of microscopy techniques with a major focus on clinical applications

    Aims and Scope

    This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard.

    Part I: Basics
    Brief history of fluorescence lifetime imaging
    The long journey to the laser and its use for nonlinear optics
    Advanced TCSPC-FLIM techniques
    Ultrafast lasers in biophotonics

    Part II: Modern nonlinear microscopy of live cells
    STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities
    Principles and applications of temporal-focusing wide-field two-photon microscopy
    FLIM-FRET microscopy
    TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing
    Laser tweezers are sources of two-photon effects
    Metabolic shifts in cell proliferation and differentiation
    Femtosecond laser nanoprocessing
    Cryomultiphoton imaging

    Part III: Nonlinear tissue imaging
    Multiphoton Tomography (MPT)
    Clinical multimodal CARS imaging
    In vivo multiphoton microscopy of human skin
    Two-photon microscopy and fluorescence lifetime imaging of the cornea
    Multiscale correlative imaging of the brain
    Revealing interaction of dyes and nanomaterials by multiphoton imaging
    Multiphoton FLIM in cosmetic clinical research
    Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery
    Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models
    Bedside assessment of multiphoton tomography


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    Karsten König, Saarland University, Germany.

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