Osteogenic lineage commitment of hMSCs on AZ91D and AZ91D coated with HA was analyzed by ALP quantification on day 1 (A) and day 14 (B) normalized to total DNA. Cell adhesion was evaluated on day 1 (C) and day 14 (D) by fluorescence staining for actin (red) and vinculin (green) that are components of focal adhesions. Tissue culture polystyrene (TCPS) was used as control. Cells were either kept in proliferation medium (control) or in osteogenic medium (osteogenic). Data are shown as mean±SD whereas * denotes significant differences with *p≤0.05 and **p≤0.01. n=3.