Figure 3:

Analysis of cell adhesion and osteogenic differentiation by ALP quantification on day 1 (A) and day 14 (B) on titanium and n-HA modified titanium. As control samples served tissue culture polystyrene (TCPS). Cell adhesion was detected on day 1 (C) and day 14 (D) by fluorescence staining for actin (red) and vinculin (green) as parts of focal adhesion complexes. Cells were either cultured in proliferation medium (control) or in osteogenic medium (osteogenic). Data are shown as mean±SD whereas * denotes significant differences with *p≤0.05 and **p≤0.01. n=3.

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