Electron microscopy of TCs in culture releasing MVC.
(A) Gathered endomembrane-bound vesicles released into the extracellular space as a cargo shielded by plasma membrane. (B) Dissolution of external membrane of MVC release individual or grouped endomembrane vesicles into the extracellular space. Electron tomography of MVCs: (C) Central tomogram section of an MVC, in direct contact with the cell membrane, revealing clustered vesicles enclosed by a membrane. (D) 3D model of section shown in (C) with isosurface representation of contained vesicles, revealing dense packaging. Cell membrane shown in dark blue. Scale bar represents 400 nm. Reproduced with permission from ref. (66).