Figure 3

Chaperone activity of lens protein. The chaperone activity of water-soluble lens fractions was measured by a light scattering of ALDH at 360 nm, following the rat lens (day 6) administration of selenite or placebo. Curve 1 represents the light scattering of ALDH in the absence of lens protein. Curve 2 represents the light scattering of mixture of G3 lens protein (selenite treatment and no hesperetin treatment) and ALDH. Curve 3 shows the light scattering of mixture of G4 lens protein (selenite and hesperetin treatment) and ALDH. Curve 4 shows the light scattering of mixture of G2 lens protein (no selenite and hesperetin treatment) and ALDH. Curve 5 was shown the light scattering of mixture of G1 lens protein (no selenite and no hesperetin treatment) and ALDH. Curve 6 was shown the light scattering of lens protein without ALDH. Increasing of light scatting indicates the ALDH aggregation, and inhibition of light scattering depends on chaperone activity.

© De Gruyter